Their ability to efficiently couple amine functionalities under mild conditions has, for many, offset the problems of ligand leaching and ionic properties associated with this chemistry. Historically, cyanogen bromide activated matrices have been very popular. Ligand characteristics will be a major determinant in choosing an appropriate matrix.
AFFINITY CHROMATOGRAPHY FREE
The ligand, generally, must possess a free primary amine, sulfhydryl, or hydroxyl group for direct attachment. We offer a variety of activated matrices ready for direct coupling to a diversity of ligands. Pires and M.C.S.The availability of pre-activated supports allows the user to quickly and easily custom synthesize affinity resins without having to handle highly reactive reagents. Affinity-Based Methods for the Separation of Parasite Proteinsīy C.R. The Value of Fungal Protease Inhibitors in Affinity Chromatographyīy Jerica Sabotič, Katarina Koruza, Boštjan Gabor, Matjaž Peterka, Miloš Barut, Janko Kos and Jože Brzin Affinity Chromatography as a Tool for Quantification of Interactions Between Drug Molecules and Their Protein Targetsīy Piotr Draczkowski, Dariusz Matosiuk and Krzysztof Jozwiak Identification of cGMP-Kinase Complexes by Affinity Chromatographyīy Salb Katharina, Schinner Elisabeth and Schlossmann Jens Affinity Chromatography as a Key Tool to Purify Protein Protease Inhibitors from Plantsīy Elizeu Antunes dos Santos, Adeliana Silva de Oliveira, Luciana Maria Araújo Rabêlo, Adriana Ferreira Uchôa and Ana Heloneida Araújo Morais Novel Detection Methods Used in Conjunction with Affinity Chromatography for the Identification and Purification of Hydrolytic Enzymes or Enzyme Inhibitors from Insects and Plantsīy Alexander V. Polyhistidine Affinity Chromatography for Purification and Biochemical Analysis of Fungal Cell Wall-Degrading Enzymes Ion Metallic Affinity Chromatography and Purification of Bacterial Toxinīy Luciano Moura Martins and Tomomasa Yano A Study of the Glutathione Transferase Proteome of Drosophila melanogaster: Use of S- Substituted Glutathiones as Affinity Ligandsīy Ramavati Pal, Milana Blakemore, Michelle Ding and Alan G. Affinity Chromatography for Purification of IgG from Human Plasmaīy Lucia Hofbauer, Leopold Bruckschwaiger, Harald Arno Butterweck and Wolfgang Teschner Immunoaffinity Chromatography: A Reviewīy Daad A. The Difference of Lectin Recovery by Sugar-Bound Resinīy Mitsuru Jimbo, Shin Satoh, Hirofumi Hasegawa, Hiroshi Miyake, Takao Yoshida, Tadashi Maruyama and Hisao Kamiya Affinity Chromatography of Lectinsīy Jure Pohleven, Borut Štrukelj and Janko Kos Affinity Interactions as a Tool for Protein Immobilization Affinity Chromatography: Principles and Applications We have always been endeavor to keep the contents of the book crisp and easily comprehensive, hoping that this book will receive an overwhelming interest, deliver benefits and valuable information to the readers. Within this book, the authors tried to deliver for you simplified fundamentals of affinity chromatography together with exemplarily applications of this versatile technique. This coined terminology covers a myriad of separation approaches that relies mainly on reversible adsorption of biomolecules through biospecific interactions on the ligand. Most will agree that one major achievement in the bio-separation techniques is affinity chromatography. Within this book, the authors tried to deliver for you simplified fundamentals of affinity chromatography together with exe.